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KMID : 0368419900330010073
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Journal of Plant Biology
1990 Volume.33 No. 1 p.73 ~ p.79
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Subcellular Distribution of Arginase in Leaves of Canavalia lineata
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Abstract
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Subcellular distribution of arginase activity was measured in leaves of Canavalia lineata. Both mitochondrial and cytosolic fraction were found to contain the arginase activity. It was noticible that cytosolic fraction contained a substantial amount of arginase activity. Different mobility of arginase from two fractions was showed on DEAE-Sephacel chromatography and polyacrylamide gel electrophoresis. Also different pI value was showed 6.3 in cytosolic and 6.7, 7.1 in mitochondrial fraction on IEF gel electrophoresis. However, canavanine-dependent-activity (CDA) of arginase in these two fractions were not different. These results indicate that heterogenity of arginase occurs in leaves of C. lineata.
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